Miss Yanyan Chen


Unsaturated lipids containing single or more carbon-carbon double bonds (C═C) within tissues are closely associated with various types of diseases. Mass spectrometry imaging (MSI) has been used to study the spatial distribution of lipid C═C location isomers on tissue sections. However, comprehensive characterization of lipid C═C location isomers using MSI remains challenging. Herein, we established an on-tissue charge-tagging Paternò−Büchi (PB) derivatization method using 3-acetylpyridine (3-AP) as a reaction reagent, which can be used to detect and assign C═C location of glycerophospholipids (GPLs) as well as neutral lipids such as fatty acids (FAs) under the same experimental workflow using matrix-assisted laser desorption/ionization (MALDI)-MSI. High coverage of mono- and poly-unsaturated C═C location isomers among various lipid classes including FA, phosphatidylcholine (PC) and sulfatide (SHexCer) in distinct regions of mouse brain and kidney were visualized using MALDI-MS/MS imaging. This method has also been applied to map the spatial distribution of lipid C═C location isomers in the Alzheimer’s disease (AD) mice model for the first time, which provides a new tool to study the relationships between the distribution of lipid structural diversity and neurodegenerative diseases.


  • Cao, W.; Cheng, S.; Yang, J.; Feng, J.; Zhang, W.; Li, Z.; Chen, Q.; Xia, Y.; Ouyang, Z.; Ma, X. Nat Commun 2020, 11, 375.
  • Harris, R. A.; May, J. C.; Stinson, C. A.; Xia, Y.; McLean, J. A. Anal Chem 2018, 90, 1915-1924.

University: HKBU

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