Abstract

Many methods have been applied to study the interactions between cells and within cells. Such as BioID, APEX and TurboID, etc.1,2 These methods generally rely on fusion proteins to achieve the function of proximity labeling. Here we use a photosensitizer to identify molecular interactions based on a proximity labeling strategy. We improved a methyl blue-based photosensitizer to achieve two aims: specific locations in cells and produce singlet oxygen (1O2). After incubation with cells, we use propargyl amine (PA) which can react with oxidized guanosines or amine acid, the propargyl amine alkyne handle also can be used react with azide-biotin and downstream study. We anticipate that our tool can help people better analyze molecular interactions in the cells.

Reference:

1. Cho, K. F.; Branon, T. C.; Udeshi, N. D.; Myers, S. A.; Carr, S. A.; Ting, A. Y. Proximity Labeling in Mammalian Cells with TurboID and Split-TurboID. Nat. Protoc. 2020, 15 (12), 3971–3999.
2. Yang, X.; Wen, Z.; Zhang, D.; Li, Z.; Li, D.; Nagalakshmi, U.; Dinesh-Kumar, S. P.; Zhang, Y. Proximity Labeling: An Emerging Tool for Probing in Planta Molecular Interactions. Plant Commun. 2021, 2 (2), 100137.